12  structures 1308  species 1922  sequences

Family: ZMP-ZTP (RF01750)

Description: ZMP/ZTP riboswitch


Wikipedia annotation Edit Wikipedia article

The Rfam group coordinates the annotation of Rfam families in Wikipedia. This family is described by a Wikipedia entry Pfl RNA motif. More...

This page is based on a Wikipedia article. The text is available under the Creative Commons Attribution/Share-Alike License.



There are various ways to view or download the seed alignments that we store. You can use a sequence viewer to look at them, or you can look at a plain text version of the sequence in a variety of different formats. More...

View options

You can view Rfam seed alignments in your browser in various ways. Choose the viewer that you want to use and click the "View" button to show the alignment in a pop-up window.


Formatting options

You can view or download Rfam seed alignments in several formats. Check either the "download" button, to save the formatted alignment, or "view", to see it in your browser window, and click "Generate".

Alignment format:


Download a gzip-compressed, Stockholm-format file containing the seed alignment for this family. You may find RALEE useful when viewing sequence alignments.

Submit a new alignment

We're happy receive updated seed alignments for new or existing families. Submit your new alignment and we'll take a look.

Secondary structure

This section shows a variety of different secondary structure representations for this family. More...

You can view the secondary structure of the family using the VARNA applet. You can see more information about VARNA iself here.

Species distribution

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Colour assignments

Archea Archea Eukaryota Eukaryota
Bacteria Bacteria Other sequences Other sequences
Viruses Viruses Unclassified Unclassified
Viroids Viroids Unclassified sequence Unclassified sequence


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This visualisation provides a simple graphical representation of the distribution of this family across species. You can find the original interactive tree in the adjacent tab. More...

Tree controls


The tree shows the occurrence of this RNA across different species. More...


Please note: for large trees this can take some time. While the tree is loading, you can safely switch away from this tab but if you browse away from the family page entirely, the tree will not be loaded.


This page displays the predicted phylogenetic tree for the alignment. More...

This tree was built using the fasttree method.

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Note: You can also download the data file for the seed tree.


For those sequences which have a structure in the Protein DataBank, we generate a mapping between EMBL, PDB and Rfam coordinate systems. The table below shows the structures on which the ZMP-ZTP family has been found.

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Motif matches

There are 1 motifs which match this family.

This section shows the Rfam motifs that match sequences within the seed alignment of this family. Users should be aware that the motifs are structural constructs and do not necessarily conform to taxonomic boundaries in the way that Rfam families do. More...

Original order Motif Accession Motif Description Number of Hits Fraction of Hits Sum of Bits Image
7 RM00008 GNRA tetraloop 21 0.119 200.8 Match Image


This section shows the database cross-references that we have for this Rfam family.

Literature references

  1. Weinberg Z, Wang JX, Bogue J, Yang J, Corbino K, Moy RH, Breaker RR; Genome Biol. 2010;11:R31. Comparative genomics reveals 104 candidate structured RNAs from bacteria, archaea and their metagenomes. PUBMED:20230605

  2. Kim PB, Nelson JW, Breaker RR Mol Cell. 2015;57:317-328. An ancient riboswitch class in bacteria regulates purine biosynthesis and one-carbon metabolism. PUBMED:25616067

  3. Jones CP, Ferre-D'Amare AR Nat Struct Mol Biol. 2015;22:679-685. Recognition of the bacterial alarmone ZMP through long-distance association of two RNA subdomains. PUBMED:26280533

  4. Kim PB, Nelson JW, Breaker RR Mol Cell. 2015;57:317-328. An ancient riboswitch class in bacteria regulates purine biosynthesis and one-carbon metabolism. PUBMED:25616067

  5. Trausch JJ, Marcano-Velazquez JG, Matyjasik MM, Batey RT Chem Biol. 2015;22:829-837. Metal Ion-Mediated Nucleobase Recognition by the ZTP Riboswitch. PUBMED:26144884

  6. Ren A, Rajashankar KR, Patel DJ Structure. 2015;23:1375-1381. Global RNA Fold and Molecular Recognition for a pfl Riboswitch Bound to ZMP, a Master Regulator of One-Carbon Metabolism. PUBMED:26118534

  7. Jones C, Tran B, Conrad C, Stagno J, Trachman R 3rd, Fischer P, Meents A, Ferre-D'Amare A Acta Crystallogr F Struct Biol Commun. 2019;75:496-500. Co-crystal structure of the Fusobacterium ulcerans ZTP riboswitch using an X-ray free-electron laser. PUBMED:31282869

  8. Tran B, Pichling P, Tenney L, Connelly CM, Moon MH, Ferre-D'Amare AR, Schneekloth JS Jr, Jones CP Cell Chem Biol. 2020;27:1241-1249. Parallel Discovery Strategies Provide a Basis for Riboswitch Ligand Design. PUBMED:32795418

External database links

Curation and family details

This section shows the detailed information about the Rfam family. We're happy to receive updated or improved alignments for new or existing families. Submit your new alignment and we'll take a look.


Seed source Published; PMID:20230605;
Structure source Published; PMID:20230605;
Type Cis-reg; riboswitch;
Author Weinberg ZORCID logo, Ontiveros-Palacios NORCID logo
Alignment details
Alignment Number of
full 1,737
seed 185

Model information

Build commands
cmbuild -F CM SEED
cmcalibrate --mpi CM
Search command
cmsearch --cpu 4 --verbose --nohmmonly -T 30.00 -Z 742849.287494 CM SEQDB
Gathering cutoff 41.0
Trusted cutoff 41.0
Noise cutoff 40.9
Covariance model Download