{"rfam": {"acc": "RF00059", "id": "TPP", "description": "TPP riboswitch aptamer (THI element)", "comment": "Vitamin B(1) in its active form thiamin pyrophosphate (TPP) is an essential coenzyme that is synthesised by coupling of pyrimidine and thiazole moieties in bacteria. The previously detected thiamin-regulatory element, thi box [1] was extended, resulting in a new, highly conserved RNA secondary structure, the THI element, which is widely distributed in eubacteria and also occurs in some archaea. Analysis of operon structures identified a large number of new candidate thiamin-regulated genes, mostly transporters, in various prokaryotic organisms [2]. The THI element is a riboswitch [3] that directly binds to TPP to regulate gene expression through a variety of mechanisms in archaea, bacteria and eukaryotes [4,5]. through a variety of mechanisms in archaea, bacteria and eukaryotes [4,5]. Tandem TPP riboswitches have been reported [6] where both TPP riboswitches respond independently to TPP. The structure of TPP riboswitch has been reported for Escherichia coli and Arabidopsis thaliana [7-12].", "curation": {"author": "Rodionov DA, Vitreshchak AG, Mironov AA, Gelfand MS, Bateman A, Moxon SJ, Ontiveros-Palacios N", "seed_source": "Vitreshchak A", "num_seed": 115, "num_full": 16130, "num_species": 8693, "type": "Cis-reg; riboswitch;", "structure_source": "Published; PMID:12376536;"}, "cm": {"build_command": "cmbuild -F CM SEED", "calibrate_command": "cmcalibrate --mpi CM", "search_command": "cmsearch --cpu 4 --verbose --nohmmonly -T 30.00 -Z 2958934 CM SEQDB", "cutoffs": {"gathering": 54.3, "trusted": 54.3, "noise": 54.2}}, "release": {"date": "2026-01-16", "number": "15.1"}, "clan": {"acc": null, "id": null}}}