{"rfam": {"acc": "RF00504", "id": "Glycine", "description": "Glycine riboswitch aptamer", "comment": "This family represents the bacterial glycine riboswitch. Glycine riboswitches usually consist of two metabolite-binding aptamers domains with similar structures in tandem. The aptamers cooperatively bind glycine to regulate the expression of downstream genes. In Bacillus subtilis, this riboswitch is found upstream of the gcvT operon which controls glycine degradation. It is thought that when glycine is in excess it will bind to both aptamers to activate these genes and facilitate glycine degradation [1].\u00c2\u00a0In vivo experiments demonstrated that glycine does not need to bind both aptamers for regulation. Mutation to the first aptamer caused greatest reduction in downstream gene expression, while mutation to the second one had varying effects. Glycine-induced expression of the gcvT operon is needed for B. subtilise growth, swarming motility and biofilm formation (in high glycine enviroment).[2]", "curation": {"author": "Moxon SJ", "seed_source": "Barrick JE, Breaker RR", "num_seed": 44, "num_full": 9425, "num_species": 5228, "type": "Cis-reg; riboswitch;", "structure_source": "Predicted; Barrick JE, Breaker RR"}, "cm": {"build_command": "cmbuild -F CM SEED", "calibrate_command": "cmcalibrate --mpi CM", "search_command": "cmsearch --cpu 4 --verbose --nohmmonly -T 30.00 -Z 2958934 CM SEQDB", "cutoffs": {"gathering": 32.0, "trusted": 32.0, "noise": 31.9}}, "release": {"date": "2026-01-16", "number": "15.1"}, "clan": {"acc": "CL00125", "id": "Glycine_riboswitch"}}}