{"rfam": {"acc": "RF00522", "id": "PreQ1", "description": "PreQ1 (pre queuosine) riboswitch aptamer", "comment": "This family is a bacterial riboswitch that binds to the metabolite preQ1, an intermediate in the biosynthesis of the modified nucleoside queuosine from GTP. One example of this riboswitch is located in the leader of the Bacillus subtilis ykvJKLM (queCDEF) operon which encodes four genes necessary for queuosine production [1]. PreQ1 binding to the riboswitch aptamer is thought to induce premature transcription termination within the leader to down-regulate expression of these genes. The 3D structure of PreQ1 riboswitch was reported in PDB 2L1V, 3FU2, 3K1V, 3GCA, 3Q50, 6VUI, 6VUH, 7E9I, 7E9E, 6E1S, 6E1T, 6E1U, 6E1V, 6E1W, 7REX and 8FB3 [2-10]. Methylation activity was reported for PreQ1 riboswitch [11].", "curation": {"author": "Barrick JE, Breaker RR, Ontiveros-Palacios N", "seed_source": "Barrick JE", "num_seed": 43, "num_full": 873, "num_species": 692, "type": "Cis-reg; riboswitch;", "structure_source": "Predicted; Barrick JE"}, "cm": {"build_command": "cmbuild -F CM SEED", "calibrate_command": "cmcalibrate --mpi CM", "search_command": "cmsearch --cpu 4 --verbose --nohmmonly -T 30.00 -Z 2958934 CM SEQDB", "cutoffs": {"gathering": 39.0, "trusted": 39.0, "noise": 38.9}}, "release": {"date": "2026-01-16", "number": "15.1"}, "clan": {"acc": null, "id": null}}}