{"rfam": {"acc": "RF02348", "id": "tracrRNA", "description": "Trans-activating crRNA", "comment": "RNA-guided Streptococcus pyogenes Cas9 (SpCas9) is a sequence-specific DNA endonuclease, the Trans-activating CRISPR RNA form an hybrid with the crRNA (crRNA/tracrRNA). The RNA hybrid acts as a guide for the endonuclease Cas9 [1,2]. The tracrRNA component structure has been reported in PDB: 6MCB and 7V59 [3,4] ", "curation": {"author": "Eberhardt R", "seed_source": "Published; PMID:22745249", "num_seed": 37, "num_full": 41, "num_species": 77, "type": "Gene; CRISPR;", "structure_source": "Predicted; PMID:22745249"}, "cm": {"build_command": "cmbuild -F CM SEED", "calibrate_command": "cmcalibrate --mpi CM", "search_command": "cmsearch --cpu 4 --verbose --nohmmonly -T 30.00 -Z 2958934 CM SEQDB", "cutoffs": {"gathering": 70.0, "trusted": 70.0, "noise": 69.7}}, "release": {"date": "2026-01-16", "number": "15.1"}, "clan": {"acc": null, "id": null}}}