# STOCKHOLM 1.0
#=GF ID mir-16
#=GF AC RF00254
#=GF DE mir-16 microRNA precursor family
#=GF AU Daub J; 0000-0001-6022-563X
#=GF GA 36.5
#=GF NC 36.4
#=GF TC 36.5
#=GF SE Griffiths-Jones SR
#=GF SS Predicted; PFOLD
#=GF TP Gene; miRNA;
#=GF BM cmbuild -F CM SEED
#=GF CB cmcalibrate --mpi CM
#=GF SM cmsearch --cpu 4 --verbose --nohmmonly -T 30.00 -Z 2958934 CM SEQDB
#=GF CL CL00085
#=GF DR MIPF; MIPF0000006;
#=GF DR SO; 0001244; pre_miRNA;
#=GF DR GO; 0035195; miRNA-mediated post-transcriptional gene silencing;
#=GF DR GO; 0016442; RISC complex;
#=GF RN [1]
#=GF RM 12434020
#=GF RT Frequent deletions and down-regulation of micro- RNA genes miR15 and miR16
#=GF RT at 13q14 in chronic lymphocytic leukemia.
#=GF RA Calin GA, Dumitru CD, Shimizu M, Bichi R, Zupo S, Noch E, Aldler H, Rattan
#=GF RA S, Keating M, Rai K, Rassenti L, Kipps T, Negrini M, Bullrich F, Croce CM
#=GF RL Proc Natl Acad Sci U S A 2002;99:15524-15529.
#=GF RN [2]
#=GF RM 32220645
#=GF RT Structural Basis for pri-miRNA Recognition by Drosha.
#=GF RA Jin W, Wang J, Liu CP, Wang HW, Xu RM
#=GF RL Mol Cell. 2020;78:423-433.
#=GF RN [3]
#=GF RM 32220646
#=GF RT Cryo-EM Structures of Human Drosha and DGCR8 in Complex with Primary
#=GF RT MicroRNA.
#=GF RA Partin AC, Zhang K, Jeong BC, Herrell E, Li S, Chiu W, Nam Y
#=GF RL Mol Cell. 2020;78:411-422.
#=GF CC miR-16, miR-15, mir-195 and miR-457 are related miRNA precursor sequences
#=GF CC (MIPF:MIPF000006). This miRNA family appears to be vertebrate specific and
#=GF CC members of this family have been predicted or experimentally validated in
#=GF CC a wide range of vertebrate species (MIPF:MIPF0000006). miR-16 and miR-15a
#=GF CC are clustered within 0.5 kb at 13q14 in human, a region shown to be
#=GF CC deleted or down-regulated in more than half of B cell chronic lymphocytic
#=GF CC leukaemias (CLL) [1]. The hairpin precursors (represented here) are
#=GF CC predicted based on base pairing and cross-species conservation -- their
#=GF CC extents are not known. In this case the mature sequence is excised from
#=GF CC the 5' arm of the hairpin. The structure of mir-16 has been reported in
#=GF CC PDB: 6LXD, 6V5B and 6V5C [2,3].
#=GF WK mir-16_microRNA_precursor_family
#=GF SQ 14

AL669869.10/100495-100571                -----------UCCUGGCUCUAGCAGCACAGAAAUAUUGGCAUGGGGAAGUGAGUCUG-----CCAAUAUUGGCUGUGCUGCUCCAGGCAGGG-------------
AY865955.1/411-487                       -----------CCUGGGCUCUAGCAGCACAGAAAUAUUGGCACAGGGAAGCGAGUCUG-----CCAAUAUUGGCUGUGCUGCUCCAGGCAGGG-------------
AL606727.3/61372-61292                   -----------GCCUGGCUGUAGCAGCACGUAAAUAUUGGAGUCAAAGCACUUGCGAAU-CCUCCAGUAUUGACCGUGCUGCUGGAGUUAGGC-------------
CR478286.10/109802-109722                -----------UCCUCGCUUUAGCAGCACGUAAAUAUUGGUGUGUUAUAGUCAAGGCCAA-CCCCAAUAUUAUGUGUGCUGCUUCAGUAAGGC-------------
AC154660.2/77731-77651                   -----------GCGGUGCCUUAGCAGCACGUAAAUAUUGGCGUUAAGAUUCUGAAAUUA-CCUCCAGUAUUGACUGUGCUGCUGAAGUAAGGU-------------
AY866308.1/534-614                       -----------GCAGUGCCUUAGCAGCACGUAAAUAUUGGCGCUAAGAUUCUAAAAUUAU-CUCCAGUAUUAACUGUGCUGCUGAAGUAAGGU-------------
AF480551.1/1-81                          -----------GCAGUGCCUUAGCAGCACGUAAAUAUUGGCGUUAAGAUUCUAAAAUUAU-CUCCAGUAUUAACUGUGCUGCUGAAGUAAGGU-------------
AY865836.1/531-611                       -----------GUUCCACUCUAGCAGCACGUAAAUAUUGGCGUAGUGAAAUAUGUAUUAAACACCAAUAUU-ACUGUGCUGCUUCAGUGUGAC-------------
AY865833.1/553-633                       -----------GUUCCACUCUAGCAGCACGUAAAUAUUGGCGUAGUGAAAUAUAUGUUAAACACCAAUAUU-ACUGUGCUGCUUUAGUGUGAC-------------
AY865835.1/569-649                       -----------GUUCCACUCUAGCAGCACGUAAAUAUUGGCGUAGUGAUAUAUAUAUUAAACACCAAUAUU-ACUGUGCUGCUUUAGUGUGAC-------------
AF480552.1/1-81                          -----------GUUCCACUCUAGCAGCACGUAAAUAUUGGCGUAGUGAAAUAUAUAUUAAACACCAAUAUU-ACUGUGCUGCUUUAGUGUGAC-------------
AC119847.12/19552-19632                  -----------GUUCCACUCUAGCAGCACGUAAAUAUUGGCGUAGUGAAAUAAAUAUUAAACACCAAUAUU-AUUGUGCUGCUUUAGUGUGAC-------------
URS0001A16A8F_9606/6-102                 UAGCAAUGUCAGCAGUGCCUUAGCAGCACGUAAAUAUUGGCC-AUUGCACU-----C---CGGCCAGUAUUAACUGUGCUGCUGAAGUAAGGUUGACCAUACUCUA
#=GR URS0001A16A8F_9606/6-102  6LXD_D_SS ((((...((((((.((((.((.(((((((((.((...................................)).)..)))))))))).)))).)))).))....))))
URS0001A16A6B_9606/1-105                 CUGACAUACUUGUUCCACUCUAGCAGCACGUAAAUAUUGGCGUAGUGAAAUAUAUAUUAAACACCAAUAUU-ACUGUGCUGCUUUAGUGUGACAGGGAUACAGCAA
#=GR URS0001A16A6B_9606/1-105  6V5B_D_SS ..(.....(((((((((((..((((((((.(.((((((((.......................)))))))).)..))))))))..)))))))))))....).....
#=GR URS0001A16A6B_9606/1-105  6V5C_D_SS ........((((((.((((..((((((((.(.((((((((.......................)))))))).)..))))))))..)))).))))))..........
#=GC SS_cons                             ...........<<<-<<<--<<<<<<<<<-<-<<<<<<<<_______________________>>>>>>>>->-->>>>>>>>-->>>>->>>.............
#=GC RF                                  ...........gccucaCUuUAGCAGCACGuAAAUAuUGGCgUaguGaaauaaauaUuAaaCaCCAaUAUUaaCUGUGCUGCUuuAGugaggc.............
//