{"rfam": {"acc": "RF00519", "id": "suhB", "description": "Makes More Granules Regulator RNA (mmgR)", "comment": "SuhB later renamed mmgR (makes more granules regulator) was a putative non-coding RNA found multiple times in the Agrobacterium tumefaciens genome and related alpha-proteobacteria. [1] Other non-coding RNAs uncovered in the same analysis include: speF, ybhL, metA, and serC. [1]Several studies inSinorhizobium meliloti showed that suhB element is indeed a non-coding RNA:firstly it was detected on Northern blot and called Sm8RNA[2], secondly in an RNAseq study referred to as SmelC689.[3]The mutant phenotype's (strain lacking the small RNA) cytoplasm contains higher content of polyhydroxybutyrate (PBH)storage granules than wild type strain. The sRNA is required to limit the PBH intracellular accumulation when the nitrogen-fixing Sinorhizobium meliloti is under condition of surplus carbon in relation to nitrogen. It has been renamed to mmgR. Further study confirmed that mmgR fine-tunes the regulation of PBH storage.[4]Northern blot confirmed the expression of the sRNA in other rhizobia species. MmgR homologues were found in most alpha-proteobacteria. The Rho-independent terminator and a single stranded region 10-mer (UUUCCUCCCU)are completely conserved. Hence, it was proposed to define a new family of alpha-proteobacterial sRNA: alpha-r8 of which mmgR is a member.[5] RNA binding protein Hfq is binding and stabilising mmgR.[6][7] Expression of the mmgR gene was shown to be controlled by nitrogen (N).[8] Further study[9] has shown that the regulatory proteins NtrC may be required for expression of mmgR gene upon N limitation. Under carbon (C) limitation, mmgR transcription is repressed by AntiA.[9]", "curation": {"author": "Barrick JE", "seed_source": "Barrick JE", "num_seed": 87, "num_full": 1791, "num_species": 918, "type": "Gene; sRNA;", "structure_source": "Predicted; Barrick JE"}, "cm": {"build_command": "cmbuild -F CM SEED", "calibrate_command": "cmcalibrate --mpi CM", "search_command": "cmsearch --cpu 4 --verbose --nohmmonly -T 19.00 -Z 2958934 CM SEQDB", "cutoffs": {"gathering": 41.0, "trusted": 41.0, "noise": 40.9}}, "release": {"date": "2026-01-16", "number": "15.1"}, "clan": {"acc": "CL00108", "id": "suhB"}}}