{"rfam": {"acc": "RF00166", "id": "PrrB_RsmZ", "description": "PrrB/RsmZ RNA family", "comment": "PrrB RNA is able to phenotypically complement gacS and gacA mutants and is itself regulated by the GacS-GacA two-component signal transduction system [1]. Inactivation of the prrB gene in Pseudomonas fluorescens F113 resulted in a significant reduction of 2, 4-diacetylphloroglucinol (Phl) and hydrogen cyanide (HCN) production, while increased metabolite production was observed when prrB was overexpressed [1]. The prrB gene sequence contains a number of imperfect repeats of the consensus sequence 5'-AGGA-3', and sequence analysis predicted a complex secondary structure featuring multiple putative stem-loops with the consensus sequences predominantly positioned at the single-stranded regions at the ends of the stem-loops. This structure is similar to the CsrB and RsmB regulatory RNAs Rfam:RF00018 [1], suggesting this RNA also interacts with a CsrA-like protein. PrrB/RsmZ RNA structure has beeing reported in PDB: 2MF1, 2MF0, 7YR6 7YR6 [3,4]", "curation": {"author": "Filiatrault M, Gardner P", "seed_source": "Filiatrault M", "num_seed": 39, "num_full": 84, "num_species": 99, "type": "Gene; sRNA;", "structure_source": "Predicted; Filiatrault M, Gardner P"}, "cm": {"build_command": "cmbuild -F CM SEED", "calibrate_command": "cmcalibrate --mpi CM", "search_command": "cmsearch --cpu 4 --verbose --nohmmonly -T 30.00 -Z 2958934 CM SEQDB", "cutoffs": {"gathering": 44.0, "trusted": 44.5, "noise": 42.9}}, "release": {"date": "2026-01-16", "number": "15.1"}, "clan": {"acc": "CL00106", "id": "Csr_Rsm_clan"}}}