{"rfam": {"acc": "RF00038", "id": "PrfA", "description": "PrfA thermoregulator UTR", "comment": "In Listeria monocytogenes, virulence genes are maximally expressed at 37 degrees C, almost silent at 30 degrees C and controlled by PrfA, a transcriptional activator whose expression is thermoregulated. It has been shown that the untranslated mRNA (UTR) preceding prfA, forms a secondary structure, which masks the ribosome binding region [1]. This family also contains UTRs from other closely related bacteria. The secondary structure of this motif forms a hairpin. The Shine-Dalgarno (SD) sequence is partly masked by the most conserved stem of the secondary structure. It is thought that at 37 degrees C the SD sequence is unmasked [1].", "curation": {"author": "Bateman A", "seed_source": "Bateman A", "num_seed": 11, "num_full": 1, "num_species": 4, "type": "Cis-reg; thermoregulator;", "structure_source": "Predicted; PFOLD"}, "cm": {"build_command": "cmbuild -F CM SEED", "calibrate_command": "cmcalibrate --mpi CM", "search_command": "cmsearch --cpu 4 --verbose --nohmmonly -T 30.00 -Z 2958934 CM SEQDB", "cutoffs": {"gathering": 88.0, "trusted": 117.4, "noise": 47.4}}, "release": {"date": "2026-01-16", "number": "15.1"}, "clan": {"acc": null, "id": null}}}