{"rfam": {"acc": "RF00021", "id": "Spot_42", "description": "Spot 42 RNA", "comment": "The physiological role of Escherichia coli Spot 42 (spf) RNA has remained obscure, even though the 109-nucleotide RNA was discovered almost three decades ago. Spot 42 seems to have a regulatory role on the galactose operon [1]. It is proposed that Spot 42 acts by an antisense mechanism where the RNA binds to the galK translation initiator region [1]. Changes in Spot 42 levels are implicated in affecting the adjacent DNA polymerase I levels [2]. The Hfq protein has been shown to interact with several small regulatory RNAs in E. coli, and the protein is required for OxyS, DsrA, RprA and Spot 42 RNA regulation [3].", "curation": {"author": "Bateman A", "seed_source": "Bateman A", "num_seed": 19, "num_full": 197, "num_species": 214, "type": "Gene; sRNA;", "structure_source": "Published; PMID:12101127"}, "cm": {"build_command": "cmbuild -F CM SEED", "calibrate_command": "cmcalibrate --mpi CM", "search_command": "cmsearch --cpu 4 --verbose --nohmmonly -T 28.80 -Z 2958934 CM SEQDB", "cutoffs": {"gathering": 80.0, "trusted": 81.4, "noise": 79.7}}, "release": {"date": "2026-01-16", "number": "15.1"}, "clan": {"acc": null, "id": null}}}